Characterization of a novel format scFv×VHH single-chain biparatopic antibody against metal binding protein MtsA.

ABSTRACT: Biparatopic antibodies (bpAbs) are engineered antibodies that bind to multiple different epitopes within the same antigens. bpAbs comprise diverse formats, including fragment-based formats, and choosing the appropriate molecular format for a desired function against a target molecule is a challenging task. Moreover, optimizing the design of constructs requires selecting appropriate antibody modalities and adjusting linker length for individual bpAbs. Therefore, it is crucial to understand the characteristics of bpAbs at the molecular level. In this study, we first obtained single-chain variable fragments and camelid heavy-chain variable domains targeting distinct epitopes of the metal binding protein MtsA and then developed a novel format single-chain bpAb connecting these fragment antibod

SUBMITTER: Asano R 

PROVIDER: S-EPMC11094767 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature