¹³C-Stable isotope resolved metabolomics uncovers dynamic biochemical landscape of gut microbiome-host organ communications in mice.

ABSTRACT:

Background

Gut microbiome metabolites are important modulators of host health and disease. However, the overall metabolic potential of the gut microbiome and interactions with the host organs have been underexplored.

Results

Using stable isotope resolved metabolomics (SIRM) in mice orally gavaged with ¹³C-inulin (a tracer), we first observed dynamic enrichment of ¹³C-metabolites in cecum contents in the amino acids and short-chain fatty acid metabolism pathways. ¹³C labeled metabolites were subsequently profiled comparatively in plasma, liver, brain, and skeletal muscle collected at 6, 12, and 24 h after the tracer administration. Organ-specific and time-dependent ¹³C metabolite enrichments were observed. Carbons from the gut micro

SUBMITTER: Xiao X 

PROVIDER: S-EPMC11094917 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature