Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses.

ABSTRACT: Hepatitis B virus (HBV) infection remains a major public health problem and, in associated co-infection with hepatitis delta virus (HDV), causes the most severe viral hepatitis and accelerated liver disease progression. As a defective satellite RNA virus, HDV can only propagate in the presence of HBV infection, which makes HBV DNA and HDV RNA the standard biomarkers for monitoring the virological response upon antiviral therapy, in co-infected patients. Although assays have been described to quantify these viral nucleic acids in circulation independently, a method for monitoring both viruses simultaneously is not available, thus hampering characterization of their complex dynamic interactions. Here, we describe the development of a dual fluorescence channel detection system for pan-genotyp

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11095294 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature