A dynamic cascade DNA nanocomplex to synergistically disrupt the pyroptosis checkpoint and relieve tumor hypoxia for efficient pyroptosis cancer therapy.

ABSTRACT: Pyroptosis has attracted widespread concerns in cancer therapy, while the therapeutic efficiency could be significantly restricted by using the crucial pyroptosis checkpoint of autophagy and tumor hypoxia. Herein, a DNA nanocomplex (DNFs@ZnMn), containing cascade DNAzymes, promoter-like ZnO₂-Mn nanozymes and photosensitizers, was constructed in one pot through rolling circle amplification reactions to induce pyroptosis through disrupting autophagy. After targeting cancer cells with a high expression of H⁺ and glutathione, DNFs@ZnMn decomposed to expose DNAzymes and promoter-like ZnO₂-Mn nanozymes. Then, sufficient metal ions and O₂ were released to promote cascade DNA/RNA cleavage and relieving of tumor hypoxia. The released DNAzyme-1 self-cleave

SUBMITTER: Wang X 

PROVIDER: S-EPMC11095510 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature