Effect of composting and storage on the microbiome and resistome of cattle manure from a commercial dairy farm in Poland.

ABSTRACT: Manure from food-producing animals, rich in antibiotic-resistant bacteria and antibiotic resistance genes (ARGs), poses significant environmental and healthcare risks. Despite global efforts, most manure is not adequately processed before use on fields, escalating the spread of antimicrobial resistance. This study examined how different cattle manure treatments, including composting and storage, affect its microbiome and resistome. The changes occurring in the microbiome and resistome of the treated manure samples were compared with those of raw samples by high-throughput qPCR for ARGs tracking and sequencing of the V3-V4 variable region of the 16S rRNA gene to indicate bacterial community composition. We identified 203 ARGs and mobile genetic elements (MGEs) in raw manure. Post-treatment

SUBMITTER: Zalewska M 

PROVIDER: S-EPMC11096248 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature