Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.

ABSTRACT: Label-free detection of multiple analytes in a high-throughput fashion has been one of the long-sought goals in biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics tools that can process small volumes of sample with high throughput, and with surface chemistry that grants analyte specificity, poses a critical challenge to date. Here, we introduce an optofluidic platform that brings together state-of-the-art digital holography with PDMS microfluidics by using supported lipid bilayers as a surface chemistry building block to integrate both technologies. Specifically, this platform fingerprints heterogeneous biological nanoparticle populations via a multiplexed label-free immunoaffinity assay with single particle sensi

SUBMITTER: Stollmann A 

PROVIDER: S-EPMC11096335 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature