Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells.

ABSTRACT: Alveolar type 2 (AT2) epithelial cells are tissue stem cells capable of differentiating into alveolar type 1 (AT1) cells for injury repair and maintenance of lung homeostasis. However, the factors involved in human AT2-to-AT1 cell differentiation are not fully understood. Here, we established SFTPC^GFP and AGER^{mCherry-HiBiT} dual-reporter induced pluripotent stem cells (iPSCs), which detected AT2-to-AT1 cell differentiation with high sensitivity and identified factors inducing AT1 cell differentiation from AT2 and their progenitor cells. We also established an "on-gel" alveolar epithelial spheroid culture suitable for medium-throughput screening. Among the 274 chemical compounds, several single compounds, including LATS-IN-1, converted AT1 cells from AT2 and their proge

SUBMITTER: Ohnishi Y 

PROVIDER: S-EPMC11096435 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature