Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium.

ABSTRACT: In natural microbiomes, microorganisms interact with each other and exhibit diverse functions. Microbiome engineering, which enables bacterial knockdown, is a promising method to elucidate the functions of targeted bacteria in microbiomes. However, few methods to selectively kill target microorganisms in the microbiome without affecting the growth of nontarget microorganisms are available. In this study, we focused on the host-specific lytic ability of virulent phages and validated their potency for precise microbiome engineering. In an artificial microbiome consisting of Escherichia coli, Pseudomonas putida, Bacillus subtilis, and Lactiplantibacillus plantarum, the addition of bacteriophages infecting their respective host strains specifically reduced the numbe

SUBMITTER: Tanaka T 

PROVIDER: S-EPMC11096457 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature