Cytoplasmic Ca²⁺ influx mediates iron- and reactive oxygen species-dependent ferroptotic cell death in rice immunity.

ABSTRACT: Iron- and reactive oxygen species (ROS)-dependent ferroptosis occurs in plant cells. Ca²⁺ acts as a conserved key mediator to control plant immune responses. Here, we report a novel role of cytoplasmic Ca²⁺ influx regulating ferroptotic cell death in rice immunity using pharmacological approaches. High Ca²⁺ influx triggered iron-dependent ROS accumulation, lipid peroxidation, and subsequent hypersensitive response (HR) cell death in rice (Oryza sativa). During Magnaporthe oryzae infection, 14 different Ca²⁺ influx regulators altered Ca²⁺, ROS and Fe²⁺ accumulation, glutathione reductase (GR) expression, glutathione (GSH) depletion and lipid peroxidation, leading to ferroptotic cell death in rice.

SUBMITTER: Wang J 

PROVIDER: S-EPMC11096502 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature