Th2-skewed peripheral T-helper cells drive B-cells in allergic bronchopulmonary aspergillosis.

ABSTRACT:

Introduction

Patients with allergic bronchopulmonary aspergillosis (ABPA) suffer from repeated exacerbations. The involvement of T-cell subsets remains unclear.

Methods

We enrolled ABPA patients, asthma patients and healthy controls. T-helper type 1 (Th1), 2 (Th2) and 17 (Th17) cells, regulatory T-cells (Treg) and interleukin (IL)-21⁺CD4⁺T-cells in total or sorted subsets of peripheral blood mononuclear cells and ABPA bronchoalveolar lavage fluid (BALF) were analysed using flow cytometry. RNA sequencing of subsets of CD4⁺T-cells was done in exacerbated ABPA patients and healthy controls. Antibodies of T-/B-cell co-cultures in vitro were measured.

Results

ABPA patients had increased Th2 cells, similar numbers of Treg cells and de

SUBMITTER: Luo RG 

PROVIDER: S-EPMC11096668 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature