Genetically engineered mouse model of HPV16 E6-E7 with vaginal-cervical intraepithelial neoplasia and decreased immunity.

ABSTRACT:

Objective

To construct models of high-risk human papillomavirus (HPV) infection with precancerous lesions or cervical cancer and explore the immune function.

Methods

Using CRISPR/Cas9, the expression vector HPV16-E6-E7-Rosa26 was microinjected into fertilized eggs of C57BL/6 N mice using homologous recombination, and the F0 generation was obtained for reproduction. Then, the formation of precancerous lesions was promoted via intramuscular injection of estradiol. Presence of precancerous cervical-vaginal intraepithelial lesions, Ki67 and p16 expression levels, and CD8⁺ T cell proportions in the spleen were evaluated.

Results

Two F0 generation mice exhibited correct the homologous recombination. Seven positive mice were identified in the F1 generation.

SUBMITTER: Xiurong D 

PROVIDER: S-EPMC11096975 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature