Guidelines for reproducible analysis of adaptive immune receptor repertoire sequencing data.

ABSTRACT: Enhancing the reproducibility and comprehension of adaptive immune receptor repertoire sequencing (AIRR-seq) data analysis is critical for scientific progress. This study presents guidelines for reproducible AIRR-seq data analysis, and a collection of ready-to-use pipelines with comprehensive documentation. To this end, ten common pipelines were implemented using ViaFoundry, a user-friendly interface for pipeline management and automation. This is accompanied by versioned containers, documentation and archiving capabilities. The automation of pre-processing analysis steps and the ability to modify pipeline parameters according to specific research needs are emphasized. AIRR-seq data analysis is highly sensitive to varying parameters and setups; using the guidelines presented here, the abil

SUBMITTER: Peres A 

PROVIDER: S-EPMC11097599 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature