A Mettl16/m⁶A/mybl2b/Igf2bp1 axis ensures cell cycle progression of embryonic hematopoietic stem and progenitor cells.

ABSTRACT: Prenatal lethality associated with mouse knockout of Mettl16, a recently identified RNA N6-methyladenosine (m⁶A) methyltransferase, has hampered characterization of the essential role of METTL16-mediated RNA m⁶A modification in early embryonic development. Here, using cross-species single-cell RNA sequencing analysis, we found that during early embryonic development, METTL16 is more highly expressed in vertebrate hematopoietic stem and progenitor cells (HSPCs) than other methyltransferases. In Mettl16-deficient zebrafish, proliferation capacity of embryonic HSPCs is compromised due to G1/S cell cycle arrest, an effect whose rescue requires Mettl16 with intact methyltransferase activity. We further identify the cell-cycle transcription factor mybl2b as a directly regul

SUBMITTER: Han Y 

PROVIDER: S-EPMC11099167 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature