Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase.

ABSTRACT: Bioluminescent indicators are power tools for studying dynamic biological processes. In this study, we present the generation of novel bioluminescent indicators by modifying the luciferin molecule with an analyte-binding moiety. Specifically, we have successfully developed the first bioluminescent indicator for potassium ions (K⁺), which are critical electrolytes in biological systems. Our approach involved the design and synthesis of a K⁺-binding luciferin named potassiorin. Additionally, we engineered a luciferase enzyme called BRIPO (bioluminescent red indicator for potassium) to work synergistically with potassiorin, resulting in optimized K⁺-dependent bioluminescence responses. Through extensive validation in cell lines, primary neurons, and live mice,

SUBMITTER: Zhao S 

PROVIDER: S-EPMC11100015 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature