A series of spontaneously blinking dyes for super-resolution microscopy.

ABSTRACT: Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.

SUBMITTER: Holland KL 

PROVIDER: S-EPMC11101128 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature