Sex-biased gene expression during neural differentiation of human embryonic stem cells.

ABSTRACT: Sex differences in the developing human brain are primarily attributed to hormonal influence. Recently however, genetic differences and their impact on the developing nervous system have attracted increased attention. To understand genetically driven sexual dimorphisms in neurodevelopment, we investigated genome-wide gene expression in an in vitro differentiation model of male and female human embryonic stem cell lines (hESC), independent of the effects of human sex hormones. Four male and four female-derived hESC lines were differentiated into a population of mixed neurons over 37 days. Differential gene expression and gene set enrichment analyses were conducted on bulk RNA sequencing data. While similar differentiation tendencies in all cell lines demonstrated the robustness and r

SUBMITTER: Pottmeier P 

PROVIDER: S-EPMC11101176 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature