Revealing the pathogenesis of gastric intestinal metaplasia based on the mucosoid air-liquid interface.

ABSTRACT:

Background

Gastric intestinal metaplasia (GIM) is an essential precancerous lesion. Although the reversal of GIM is challenging, it potentially brings a state-to-art strategy for gastric cancer therapeutics (GC). The lack of the appropriate in vitro model limits studies of GIM pathogenesis, which is the issue this work aims to address for further studies.

Method

The air-liquid interface (ALI) model was adopted for the long-term culture of GIM cells in the present work. This study conducted Immunofluorescence (IF), quantitative real-time polymerase chain reaction (qRT-PCR), transcriptomic sequencing, and mucoproteomic sequencing (MS) techniques to identify the pathways for differential expressed genes (DEGs) enrichment among different groups, furthermore, to verify novel biom

SUBMITTER: Liu S 

PROVIDER: S-EPMC11101349 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature