Nanopore sequencing of influenza A and B in Oxfordshire and the United Kingdom, 2022-23.

ABSTRACT:

Objectives

We evaluated Nanopore sequencing for influenza surveillance.

Methods

Influenza A and B PCR-positive samples from hospital patients in Oxfordshire, UK, and a UK-wide population survey from winter 2022-23 underwent Nanopore sequencing following targeted rt-PCR amplification.

Results

From 941 infections, successful sequencing was achieved in 292/388 (75 %) available Oxfordshire samples: 231 (79 %) A/H3N2, 53 (18 %) A/H1N1, and 8 (3 %) B/Victoria and in 53/113 (47 %) UK-wide samples. Sequencing was more successful at lower Ct values. Most same-sample replicate sequences had identical haemagglutinin segments (124/141, 88 %); 36/39 (92 %) Illumina vs. Nanopore comparisons were identical, and 3 (8 %) differed by 1 variant. Comparison of Oxfordshire and UK-wide seq

SUBMITTER: Cane J 

PROVIDER: S-EPMC11101610 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature