NK Cell-Monocyte Cross-talk Underlies NK Cell Activation in Severe COVID-19.

ABSTRACT: NK cells in the peripheral blood of severe COVID-19 patients exhibit a unique profile characterized by activation and dysfunction. Previous studies have identified soluble factors, including type I IFN and TGF-β, that underlie this dysregulation. However, the role of cell-cell interactions in modulating NK cell function during COVID-19 remains unclear. To address this question, we combined cell-cell communication analysis on existing single-cell RNA sequencing data with in vitro primary cell coculture experiments to dissect the mechanisms underlying NK cell dysfunction in COVID-19. We found that NK cells are predicted to interact most strongly with monocytes and that this occurs via both soluble factors and direct interactions. To validate these findings, we performed in vitro cocultures i

SUBMITTER: Lee MJ 

PROVIDER: S-EPMC11102029 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature