Species-specific regulation of alternative splicing in the C-terminal region of the p53 tumor suppressor gene.

ABSTRACT: Alternative splicing occurs in the C-terminal region of the p53 tumor suppressor gene between two alternative 3' splice sites in intron 10. This alternative splicing event has been detected in murine cells, but not in rat or human tissues. In this paper, we have characterized the pattern of p53 alternative splicing in cell lines from five different species. Our results confirm that p53 alternative splicing is species-specific, being detected only in cell lines of rodent origin. Using transient transfection assays, we have established that the rat p53 gene undergoes efficient alternative splicing in both mouse and rat cell lines, thus demonstrating that it has all the necessary cis -acting sequences to be alternatively spliced. In contrast, we were unable to detect any usage of the human al

SUBMITTER: Laverdiere M 

PROVIDER: S-EPMC111041 | biostudies-literature | 2000 Mar

REPOSITORIES: biostudies-literature