Long-term in vivo three-photon imaging reveals region-specific differences in healthy and regenerative oligodendrogenesis.

ABSTRACT: The generation of new myelin-forming oligodendrocytes in the adult central nervous system is critical for cognitive function and regeneration following injury. Oligodendrogenesis varies between gray and white matter regions, suggesting that local cues drive regional differences in myelination and the capacity for regeneration. However, the layer- and region-specific regulation of oligodendrocyte populations is unclear due to the inability to monitor deep brain structures in vivo. Here we harnessed the superior imaging depth of three-photon microscopy to permit long-term, longitudinal in vivo three-photon imaging of the entire cortical column and subcortical white matter in adult mice. We find that cortical oligodendrocyte populations expand at a higher rate in the adult brain than those of

SUBMITTER: Thornton MA 

PROVIDER: S-EPMC11104262 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature