TPX2 upregulates MMP13 to promote the progression of lipopolysaccharide-induced osteoarthritis.

ABSTRACT:

Purpose

This study seeks to identify potential clinical biomarkers for osteoarthritis (OA) using bioinformatics and investigate OA mechanisms through cellular assays.

Methods

Differentially Expressed Genes (DEGs) from GSE52042 (four OA samples, four control samples) were screened and analyzed with protein-protein interaction (PPI) analysis. Overlapping genes in GSE52042 and GSE206848 (seven OA samples, and seven control samples) were identified and evaluated using Gene Set Enrichment Analysis (GSEA) and clinical diagnostic value analysis to determine the hub gene. Finally, whether and how the hub gene impacts LPS-induced OA progression was explored by in vitro experiments, including Western blotting (WB), co-immunoprecipitation (Co-IP), flow cytometry, etc.

SUBMITTER: Yu J 

PROVIDER: S-EPMC11104344 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature