Identification of ligand and receptor interactions in CKD and MASH through the integration of single cell and spatial transcriptomics.

ABSTRACT:

Background

Chronic Kidney Disease (CKD) and Metabolic dysfunction-associated steatohepatitis (MASH) are metabolic fibroinflammatory diseases. Combining single-cell (scRNAseq) and spatial transcriptomics (ST) could give unprecedented molecular disease understanding at single-cell resolution. A more comprehensive analysis of the cell-specific ligand-receptor (L-R) interactions could provide pivotal information about signaling pathways in CKD and MASH. To achieve this, we created an integrative analysis framework in CKD and MASH from two available human cohorts.

Results

The analytical framework identified L-R pairs involved in cellular crosstalk in CKD and MASH. Interactions between cell types identified using scRNAseq data were validated by checking the spatial co-presence usi

SUBMITTER: Moreno J 

PROVIDER: S-EPMC11104622 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature