Purinergic P2Y₂ receptors modulate endothelial sprouting.

ABSTRACT: Purinergic P2 receptors are critical regulators of several functions within the vascular system, including platelet aggregation, vascular inflammation, and vascular tone. However, a role for ATP release and P2Y receptor signalling in angiogenesis remains poorly defined. Here, we demonstrate that blood vessel growth is controlled by P2Y₂ receptors. Endothelial sprouting and vascular tube formation were significantly dependent on P2Y₂ expression and inhibition of P2Y₂ using a selective antagonist blocked microvascular network generation. Mechanistically, overexpression of P2Y₂ in endothelial cells induced the expression of the proangiogenic molecules CXCR4, CD34, and angiopoietin-2, while expression of VEGFR-2 was decreased. Interestingly, elevated

SUBMITTER: Muhleder S 

PROVIDER: S-EPMC11104991 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature