Genome wide inherited modifications of the tomato epigenome by trans-activated bacterial CG methyltransferase.

ABSTRACT:

Background

Epigenetic variation is mediated by epigenetic marks such as DNA methylation occurring in all cytosine contexts in plants. CG methylation plays a critical role in silencing transposable elements and regulating gene expression. The establishment of CG methylation occurs via the RNA-directed DNA methylation pathway and CG methylation maintenance relies on METHYLTRANSFERASE1, the homologue of the mammalian DNMT1.

Purpose

Here, we examined the capacity to stably alter the tomato genome methylome by a bacterial CG-specific M.SssI methyltransferase expressed through the LhG4/pOP transactivation system.

Results

Methylome analysis of M.SssI expressing plants revealed that their euchromatic genome regions are specifically hypermethylated in the CG context, and so ar

SUBMITTER: Kumar BKP 

PROVIDER: S-EPMC11106227 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature