CD4⁺ T cell heterogeneity in gestational age and preeclampsia using single-cell RNA sequencing.

ABSTRACT: A balance between pro-inflammatory decidual CD4⁺ T cells and FOXP3⁺ regulatory T cells (FOXP3⁺ Tregs) is important for maintaining fetomaternal tolerance. Using single-cell RNA-sequencing and T cell receptor repertoire analysis, we determined that diversity and clonality of decidual CD4⁺ T cell subsets depend on gestational age. Th1/Th2 intermediate and Th1 subsets of CD4⁺ T cells were clonally expanded in both early and late gestation, whereas FOXP3⁺ Tregs were clonally expanded in late gestation. Th1/Th2 intermediate and FOXP3⁺ Treg subsets showed altered gene expression in preeclampsia (PE) compared to healthy late gestation. The Th1/Th2 intermediate subset exhibited elevated levels

SUBMITTER: Tsuda S 

PROVIDER: S-EPMC11106458 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature