Characterization of arbuscular mycorrhizal fungal species associating with Zea mays.

ABSTRACT: Taxonomic identification of arbuscular mycorrhizal (AM) fungal spores extracted directly from the field is sometimes difficult because spores are often degraded or parasitized by other organisms. Single-spore inoculation of a suitable host plant allows for establishing monosporic cultures of AM fungi. This study aimed to propagate AM fungal spores isolated from maize soil using single spores for morphological characterization. First, trap cultures were established to trigger the sporulation of AM fungal species. Second, trap cultures were established with individual morphotypes by picking up only one spore under a dissecting microscope and transferring it to a small triangle of sterilized filter paper, which was then carefully inoculated below a root from germinated sorghum seeds in each p

SUBMITTER: Mausse-Sitoe S 

PROVIDER: S-EPMC11106459 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature