Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters.

ABSTRACT: Streptomyces offer a wealth of naturally occurring compounds with diverse structures, many of which possess significant pharmaceutical values. However, new product exploration and increased yield of specific compounds in Streptomyces have been technically challenging due to their slow growth rate, complex culture conditions and intricate genetic backgrounds. In this study, we screened dozens of Streptomyces strains inhabiting in a plant rhizosphere for fast-growing candidates, and further employed CRISPR/Cas-based engineering techniques for stepwise refinement of a particular strain, Streptomyces sp. A-14 that harbors a 7.47 Mb genome. After strategic removal of nonessential genomic regions and most gene clusters, we reduced its genome size to 6.13 Mb, while pre

SUBMITTER: Li H 

PROVIDER: S-EPMC11106782 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature