An HPLC-SEC-based rapid quantification method for vesicular stomatitis virus particles to facilitate process development.

ABSTRACT: Virus particle (VP) quantification plays a pivotal role in the development of production processes of VPs for virus-based therapies. The yield based on total VP count serves as a process performance indicator for evaluating process efficiency and consistency. Here, a label-free particle quantification method for enveloped VPs was developed, with potential applications in oncolytic virotherapy, vaccine development, and gene therapy. The method comprises size-exclusion chromatography (SEC) separation using high-performance liquid chromatography (HPLC) instruments. Ultraviolet (UV) was used for particle quantification and multi-angle light scattering (MALS) for particle characterization. Consistent recoveries of over 97% in the SEC were achieved upon mobile phase screenings and addition of bo

SUBMITTER: Schimek A 

PROVIDER: S-EPMC11107205 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature