Dicer generates a regulatory microRNA network in smooth muscle cells that limits neointima formation during vascular repair.

ABSTRACT: MicroRNAs (miRNAs) coordinate vascular repair by regulating injury-induced gene expression in vascular smooth muscle cells (SMCs) and promote the transition of SMCs from a contractile to a proliferating phenotype. However, the effect of miRNA expression in SMCs on neointima formation is unclear. Therefore, we studied the role of miRNA biogenesis by Dicer in SMCs in vascular repair. Following wire-induced injury to carotid arteries of Apolipoprotein E knockout (Apoe ^-/-) mice, miRNA microarray analysis revealed that the most significantly regulated miRNAs, such as miR-222 and miR-21-3p, were upregulated. Conditional deletion of Dicer in SMCs increased neointima formation by reducing SMC proliferation in Apoe ^-/- mice, and decreased mainly the expression of miRNAs, such

SUBMITTER: Zahedi F 

PROVIDER: S-EPMC11107738 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature