Porin deficiency or plasmid copy number increase mediated carbapenem-resistant Escherichia coli resistance evolution.

ABSTRACT: This study investigated resistance evolution mechanisms of conjugated plasmids and bacterial hosts under different concentrations of antibiotic pressure. Ancestral strain ECNX52 was constructed by introducing the bla_NDM-5-carrying IncX3 plasmid into E. coli C600, and was subjected to laboratory evolution under different concentrations of meropenem pressure. Minimal inhibitory concentrations and conjugation frequency were determined. Fitness of these strains was assessed. Whole genome sequencing and transcriptional changes were performed. Ancestral host or plasmids were recombined with evolved hosts or plasmids to verify plasmid or host factors in resistance evolution. Role of the repA mutation on plasmid copy number was determined. Two out of the four clones

SUBMITTER: Xiang G 

PROVIDER: S-EPMC11107853 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature