Single Cell Analysis of Human Colonoids Exposed to Uranium-Bearing Dust.

ABSTRACT:

Background

Uranium exposure remains an important environmental legacy and physiological health concern, with hundreds of abandoned uranium mines located in the Southwestern United States largely impacting underserved indigenous communities. The negative effects of heavy metals on barrier permeability and inhibition of intestinal epithelial healing have been described; however, transcriptomic changes within the intestinal epithelial cells and impacts on lineage differentiation are largely unknown.

Objectives

Herein, we sought to determine the molecular and cellular changes that occur in the colon in response to uranium bearing dust (UBD) exposure.

Methods

Human colonoids from three biologically distinct donors were acutely exposed to UBD then digested for single cell R

SUBMITTER: Atanga R 

PROVIDER: S-EPMC11108582 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature