Human antibody signatures towards the Chlamydia trachomatis major outer membrane protein after natural infection and vaccination.

ABSTRACT:

Background

Chlamydia trachomatis (CT) Major Outer Membrane Protein (MOMP) holds a neutralising epitope in the Variable Domain 4 (VD4), and this region's immune dominance during infection is well known. This study aimed to assess the antibody response induced after infection and compare it for specificity and functionality to the response following vaccination with the vaccine CTH522, which contains VD4's from serovars D, E, F, and G.

Methods

We assessed the antibody epitopes in MOMP by a high density peptide array. Furthermore, the role of the VD4 epitope in neutralisation was explored by competitive inhibition experiments with a fusion protein holding the neutralising VD4 linear epitope. This was done in two independent groups: 1) MOMP seropositive individuals infected with

SUBMITTER: Rosenkrands I 

PROVIDER: S-EPMC11108849 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature