A comparative study on the dose-effect of low-dose radiation based on microdosimetric analysis and single-cell sequencing technology.

ABSTRACT: The biological mechanisms triggered by low-dose exposure still need to be explored in depth. In this study, the potential mechanisms of low-dose radiation when irradiating the BEAS-2B cell lines with a Cs-137 gamma-ray source were investigated through simulations and experiments. Monolayer cell population models were constructed for simulating and analyzing distributions of nucleus-specific energy within cell populations combined with the Monte Carlo method and microdosimetric analysis. Furthermore, the 10 × Genomics single-cell sequencing technology was employed to capture the heterogeneity of individual cell responses to low-dose radiation in the same irradiated sample. The numerical uncertainties can be found both in the specific energy distribution in microdosimetry and in differential

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11109114 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature