Discovery of fungal onoceroid triterpenoids through domainless enzyme-targeted global genome mining.

ABSTRACT: Genomics-guided methodologies have revolutionized the discovery of natural products. However, a major challenge in the field of genome mining is determining how to selectively extract biosynthetic gene clusters (BGCs) for untapped natural products from numerous available genome sequences. In this study, we developed a fungal genome mining tool that extracts BGCs encoding enzymes that lack a detectable protein domain (i.e., domainless enzymes) and are not recognized as biosynthetic proteins by existing bioinformatic tools. We searched for BGCs encoding a homologue of Pyr4-family terpene cyclases, which are representative examples of apparently domainless enzymes, in approximately 2000 fungal genomes and discovered several BGCs with unique features. The subsequent characterization of selecte

SUBMITTER: Tang J 

PROVIDER: S-EPMC11109268 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature