Ribosome rescue factor PELOTA modulates translation start site choice for C/EBPα protein isoforms.

ABSTRACT: Translation initiation at alternative start sites can dynamically control the synthesis of two or more functionally distinct protein isoforms from a single mRNA. Alternate isoforms of the developmental transcription factor CCAAT/enhancer-binding protein α (C/EBPα) produced from different start sites exert opposing effects during myeloid cell development. This choice between alternative start sites depends on sequence features of the CEBPA transcript, including a regulatory uORF, but the molecular basis is not fully understood. Here, we identify the factors that affect C/EBPα isoform choice using a sensitive and quantitative two-color fluorescent reporter coupled with CRISPRi screening. Our screen uncovered a role of the ribosome rescue factor PELOTA (PELO) in promoting the expressio

SUBMITTER: Fernandez SG 

PROVIDER: S-EPMC11109482 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature