BCMA/CD47-directed universal CAR-T cells exhibit excellent antitumor activity in multiple myeloma.

ABSTRACT:

Background

BCMA-directed autologous chimeric antigen receptor T (CAR-T) cells have shown excellent clinical efficacy in relapsed or refractory multiple myeloma (RRMM), however, the current preparation process for autologous CAR-T cells is complicated and costly. Moreover, the upregulation of CD47 expression has been observed in multiple myeloma, and anti-CD47 antibodies have shown remarkable results in clinical trials. Therefore, we focus on the development of BCMA/CD47-directed universal CAR-T (UCAR-T) cells to improve these limitations.

Methods

In this study, we employed phage display technology to screen nanobodies against BCMA and CD47 protein, and determined the characterization of nanobodies. Furthermore, we simultaneously disrupted the endogenous TRAC and B2M genes of

SUBMITTER: Lu Q 

PROVIDER: S-EPMC11112799 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature