Fermentative aminopyrrolnitrin production by metabolically engineered Corynebacterium glutamicum.

ABSTRACT: Aminopyrrolnitrin (APRN), a natural halogenated phenylpyrrole derivative (HPD), has strong antifungal and antiparasitic activities. Additionally, it showed 2.8-fold increased photostability compared to pyrrolnitrin, a commercially available HPD with antimicrobial activity. For microbial production of APRN, we first engineered anthranilate phosphoribosyltransferase encoded by trpD from Corynebacterium glutamicum, resulting in a TrpD^A162D mutation that exhibits feedback-resistant against L-tryptophan and higher substrate affinity compared to wild-type TrpD. Plasmid-borne expression of trpD^A162D in C. glutamicum TP851 strain with two copies of trpD^A162D in the genome led to the production of 3.1 g/L L-tryptophan in flask culture. Subsequent step for L-tryptoph

SUBMITTER: Putri VRM 

PROVIDER: S-EPMC11112847 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature