Cell-penetrating peptide secures an efficient endosomal escape of an intact cargo upon a brief photo-induction.

ABSTRACT: Since their discovery, cell-penetrating peptides (CPPs) have provided a novel, efficient, and non-invasive mode of transport for various (bioactive) cargos into cells. Despite the ever-growing number of successful implications of the CPP-mediated delivery, issues concerning their intracellular trafficking, significant targeting to degradative organelles, and limited endosomal escape are still hindering their widespread use. To overcome these obstacles, we have utilized a potent photo-induction technique with a fluorescently labeled protein cargo attached to an efficient CPP, TP10. In this study we have determined some key requirements behind this induced escape (e.g., dependence on peptide-to-cargo ratio, time and cargo), and have semi-quantitatively assessed the characteristics of the end

SUBMITTER: Raagel H 

PROVIDER: S-EPMC11113630 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature