Proteomic analysis of human sperm reveals changes in protamine 1 phosphorylation in men with infertility.

ABSTRACT:

Objective

To perform a comprehensive assessment of protamine (P) isoforms and modifications in human sperm with the aim of identifying how P modifications and isoforms are altered in men with reduced sperm motility and low sperm count.

Design

Cross-sectional.

Setting

Academic medical center.

Patients

A total of 18 men with prior reported pregnancy and normozoospermia (normal sperm), 14 men from couples with infertility and asthenozoospermia (reduced sperm motility), and 24 men from couples with infertility and oligoasthenoteratozoospermia (low sperm count and motility and abnormal sperm morphology).

Intervention(s)

Not applicable.

Main outcome measure(s)

Proteomic assessment using both top-down and bottom-up liquid chromatography mass spectrometry

SUBMITTER: Schon SB 

PROVIDER: S-EPMC11116066 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature