Analysis of cholesterol-recognition motifs of the plasma membrane Ca²⁺-ATPase.

ABSTRACT: The plasma membrane Ca²⁺-ATPase (PMCA) is crucial for the fine tuning of intracellular calcium levels in eukaryotic cells. In this study, we show the presence of CARC sequences in all human and rat PMCA isoforms and we performed further analysis by molecular dynamics simulations. This analysis focuses on PMCA1, containing three CARC motifs, and PMCA4, with four CARC domains. In PMCA1, two CARC motifs reside within transmembrane domains, while the third is situated at the intracellular interface. The simulations depict more stable RMSD values and lower RMSF fluctuations in the presence of cholesterol, emphasizing its potential stabilizing effect. In PMCA4, a distinct dynamic was found. Notably, the total energy differences between simulations with cholesterol and phospholipids ar

SUBMITTER: Delgado-Coello B 

PROVIDER: S-EPMC11116186 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature