Retinal microglia express more MHC class I and promote greater T-cell-driven inflammation than brain microglia.

ABSTRACT:

Introduction

Macrophage function is determined by microenvironment and origin. Brain and retinal microglia are both derived from yolk sac progenitors, yet their microenvironments differ. Utilizing single-cell RNA sequencing (scRNA-seq) data from mice, we tested the hypothesis that retinal and brain microglia exhibit distinct transcriptional profiles due to their unique microenvironments.

Methods

Eyes and brains from 2-4 month wildtype mice were combined (20 eyes; 3 brains) to yield one biologically diverse sample per organ. Each tissue was digested into single cell suspensions, enriched for immune cells, and sorted for scRNA-seq. Analysis was performed in Seurat v3 including clustering, integration, and differential expression. Multi-parameter flow cytometry was used for val

SUBMITTER: Bloomfield CL 

PROVIDER: S-EPMC11116593 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature