Kinetic Analysis of Lanthipeptide Cyclization by Substrate-Tolerant ProcM.

ABSTRACT: Lanthipeptides are ribosomally synthesized and post-translationally modified peptides characterized by the presence of thioether crosslinks. Class II lanthipeptide synthetases are bifunctional enzymes responsible for the multistep chemical modification of these natural products. ProcM is a class II lanthipeptide synthetase known for its remarkable substrate tolerance and ability to install diverse (methyl)lanthionine rings with high accuracy. Previous studies suggested that the final ring pattern of the lanthipeptide product may be determined by the substrate sequence rather than by ProcM, and that ProcM operates by a kinetically controlled mechanism, wherein the ring pattern is dictated by the relative rates of the individual cyclization reactions. This study utilizes kinetic assays to de

SUBMITTER: Desormeaux EK 

PROVIDER: S-EPMC11118578 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature