Comprehensive integration of single-cell transcriptomic data illuminates the regulatory network architecture of plant cell fate specification.

ABSTRACT: Plant morphogenesis relies on precise gene expression programs at the proper time and position which is orchestrated by transcription factors (TFs) in intricate regulatory networks in a cell-type specific manner. Here we introduced a comprehensive single-cell transcriptomic atlas of Arabidopsis seedlings. This atlas is the result of meticulous integration of 63 previously published scRNA-seq datasets, addressing batch effects and conserving biological variance. This integration spans a broad spectrum of tissues, including both below- and above-ground parts. Utilizing a rigorous approach for cell type annotation, we identified 47 distinct cell types or states, largely expanding our current view of plant cell compositions. We systematically constructed cell-type specific gene regulato

SUBMITTER: Cao S 

PROVIDER: S-EPMC11119547 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature