Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes.

ABSTRACT: In this study, we report the complexities and challenges associated with achieving robust RNA interference (RNAi)-mediated gene knockdown in the mosquitoes Aedes aegypti and Aedes albopictus, a pivotal approach for genetic analysis and vector control. Despite RNAi's potential for species-specific gene targeting, our independent efforts to establish oral delivery of RNAi for identifying genes critical for mosquito development and fitness encountered significant challenges, failing to reproduce previously reported potent RNAi effects. We independently evaluated a range of RNAi-inducing molecules (siRNAs, shRNAs, and dsRNAs) and administration methods (oral delivery, immersion, and microinjection) in three different laboratories. We also tested various mosquito strains and utili

SUBMITTER: Figueiredo Prates LH 

PROVIDER: S-EPMC11121262 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature