Novel Genome-Engineered H Alleles Differentially Affect Lateral Inhibition and Cell Dichotomy Processes during Bristle Organ Development.

ABSTRACT: Hairless (H) encodes the major antagonist in the Notch signaling pathway, which governs cellular differentiation of various tissues in Drosophila. By binding to the Notch signal transducer Suppressor of Hairless (Su(H)), H assembles repressor complexes onto Notch target genes. Using genome engineering, three new H alleles, H^FA, H^LLAA and H^WA were generated and a phenotypic series was established by several parameters, reflecting the residual H-Su(H) binding capacity. Occasionally, homozygous H^WA flies develop to adulthood. They were compared with the likewise semi-viable H^NN allele affecting H-Su(H) nuclear entry. The H homozygotes were short-lived, sterile and flightless, yet sho

SUBMITTER: Monch TC 

PROVIDER: S-EPMC11121709 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature