Synthetic ShK-like Peptide from the Jellyfish <i>Nemopilema nomurai</i> Has Human Voltage-Gated Potassium-Channel-Blocking Activity.

ABSTRACT: We identified a new human voltage-gated potassium channel blocker, NnK-1, in the jellyfish Nemopilema nomurai based on its genomic information. The gene sequence encoding NnK-1 contains 5408 base pairs, with five introns and six exons. The coding sequence of the NnK-1 precursor is 894 nucleotides long and encodes 297 amino acids containing five presumptive ShK-like peptides. An electrophysiological assay demonstrated that the fifth peptide, NnK-1, which was chemically synthesized, is an effective blocker of hKv1.3, hKv1.4, and hKv1.5. Multiple-sequence alignment with cnidarian Shk-like peptides, which have Kv1.3-blocking activity, revealed that three residues (³Asp, ²⁵Lys, and ³⁴Thr) of NnK-1, together with six cysteine residues, were conserved. Ther

SUBMITTER: Kim YJ 

PROVIDER: S-EPMC11122761 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature