Retrospective Genotyping of Enteroviruses Using a Diagnostic Nanopore Sequencing Workflow.

ABSTRACT: Enteroviruses are among the most common viruses pathogenic to humans. They are associated with various forms of disease, ranging from mild respiratory illness to severe neurological diseases. In recent years, an increasing number of isolated cases of children developing meningitis or encephalitis as a result of enterovirus infection have been reported, as well as discrete enterovirus D68 outbreaks in North America in 2014 and 2016. We developed an assay to rapidly genotype enteroviruses by sequencing a region within the VP1 gene using nanopore Flongles. We retrospectively analyzed enterovirus-/rhinovirus-positive clinical samples from the Zurich, Switzerland area mainly collected during two seasons in 2019/2020 and 2021/2022. Respiratory, cerebrospinal fluid, and stool samples were analyze

SUBMITTER: van Ackeren V 

PROVIDER: S-EPMC11124337 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature