Long-Read Nanopore-Based Sequencing of Anelloviruses.

ABSTRACT: Routinely used metagenomic next-generation sequencing (mNGS) techniques often fail to detect low-level viremia (<10⁴ copies/mL) and appear biased towards viruses with linear genomes. These limitations hinder the capacity to comprehensively characterize viral infections, such as those attributed to the Anelloviridae family. These near ubiquitous non-pathogenic components of the human virome have circular single-stranded DNA genomes that vary in size from 2.0 to 3.9 kb and exhibit high genetic diversity. Hence, species identification using short reads can be challenging. Here, we introduce a rolling circle amplification (RCA)-based metagenomic sequencing protocol tailored for circular single-stranded DNA genomes, utilizing the long-read Oxford Nanopore platform. The approac

SUBMITTER: Anantharam R 

PROVIDER: S-EPMC11125752 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature