Expression analysis of secreted and cell surface genes of five transformed human cell lines and derivative xenograft tumors.

ABSTRACT:

Background

Since the early stages of tumorigenesis involve adhesion, escape from immune surveillance, vascularization and angiogenesis, we devised a strategy to study the expression profiles of all publicly known and putative secreted and cell surface genes. We designed a custom oligonucleotide microarray containing probes for 3531 secreted and cell surface genes to study 5 diverse human transformed cell lines and their derivative xenograft tumors. The origins of these human cell lines were lung (A549), breast (MDA MB-231), colon (HCT-116), ovarian (SK-OV-3) and prostate (PC3) carcinomas.

Results

Three different analyses were performed: (1) A PCA-based linear discriminant analysis identified a 54 gene profile characteristic of all tumors, (2) Application of MANOVA (Pcorr < .

SUBMITTER: Stull RA 

PROVIDER: S-EPMC1112590 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature